Samsung RT34M Uživatelský manuál stáhnout pdf (Strana 83)

Dushyant Patel Extractive Spectrophotometric Method

S.K.P.C.P.E.R., Kherva 71 M .Pharm. Thesis

buffer solution was added in the different separating funnels. BCG solution or BTB

solution was added in excess each. Shaken well and extracted with 10 ml of chloroform.

Later the extracts were taken into 10 ml volumetric flasks, treated with anhydrous sodium

sulphate and volume was made up with chloroform, and then absorbances were

measured at 416 nm and 421 nm for BCG and BTB, respectively. It was found that after

addition of 2 ml of buffer in BCG and 2.5 ml of buffer in BTB, absorbance became

constant. Hence 2 ml and 2.5 ml of buffer solution were optimized for BCG method and

BTB method, respectively (Figure 5.2 A and B).

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0246

pHofbuffer

Absorbance

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0246

pHofbuffer

Absorbance

(A) (B)

Figure 5.1 Optimization of pH of Buffer for (A) GBP-BCG (B) GBP-BTB complex

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01234

mlOfBuffer

Absorbance

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01234

mlOfBuffer

Absorbance

(A) (B)

Figure 5.2 Optimization of volume of Buffer for (A) GBP-BCG (B) GBP-BTB complex

d) Stability study of drug dye complexes:

¾ The stability of the drug dye complexes was determined individually for both the dyes

(BCG and BTB). Working standard solution (5 ml) was pipette out and added to a

1 2 ... 78 79 80 81 82 83 84 85 86 87 88 ... 92 93

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