Samsung RT34M Uživatelský manuál stáhnout pdf (Strana 81)

Dushyant Patel Extractive Spectrophotometric Method

S.K.P.C.P.E.R., Kherva 69 M .Pharm. Thesis

¾ AR grade Bromocresol Green reagent and Bromothymol Blue reagent ((Finar Chemicals

Ltd., India)

¾ Whatman filter paper no. 41 (Millipore, USA)

5.2.3 PREPARATION OF REAGENTS:

a) Preparation of diluent:

¾ Distilled water was used as diluent.

b) Preparation of buffer solutions:

• Phosphate buffer solution (pH 2.0):

¾ Potassium dihydrogen phosphate (13.6 mg) was accurately weighed and dissolved in

water (100 ml). pH of solution was adjusted to 2.0 with HCl.

• Phosphate buffer solution (pH 2.5):

¾ Potassium dihydrogen phosphate (10 g) was accurately weighed and dissolved in water

(100 ml). pH of solution was adjusted to 2.5 with HCl.

• Phosphate buffer solution (pH 3.0):

¾ Potassium dihydrogen phosphate (13.6 mg) was accurately weighed and dissolved in

water (100 ml). pH of solution was adjusted to 3.0 with HCl.

• Phosphate buffer solution (pH 3.5):

¾ Potassium dihydrogen phosphate (13.6 mg) was accurately weighed and dissolved in

water (100 ml). pH of solution was adjusted to 3.5 with HCl.

• Phosphate buffer solution (pH 4.0):

¾ Disodium hydrogen phosphate (504 mg) and Potassium dihydrogen phosphate (301 mg)

were accurately weighed and dissolved in water (100 ml). pH of solution was adjusted to

4.0 with glacial acetic acid.

• Phosphate buffer solution (pH 4.5):

¾ Disodium hydrogen phosphate (504 mg) and Potassium dihydrogen phosphate (301 mg)

were accurately weighed and dissolved in water (100 ml). pH of solution was adjusted to

4.6 with glacial acetic acid.

• Phosphate buffer solution (pH 5.0):

¾ Potassium dihydrogen phosphate (4 g) was accurately weighed and dissolved in water

(100 ml). pH of solution was adjusted to 5.0 with 10 M KOH.

• Phosphate buffer solution (pH 5.5):

¾ Potassium dihydrogen phosphate (4 g) was accurately weighed and dissolved in water

(100 ml). pH of solution was adjusted to 5.5 with 10 M KOH.

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Samsung RT34M Uživatelský manuál Strana 81