Samsung RT34M Uživatelský manuál stáhnout pdf (Strana 52)

Dushyant Patel LC-MS/MS Method

S.K.P.C.P.E.R., Kherva 40 M .Pharm. Thesis

4.2.12 SAMPLE TREATMENT/SAMPLE PREPARATION:

¾ The study samples along with calibration standards and QC samples were taken out from

the -20°C/-70°C deep freezer and kept at room temperature for thawing. Vortexed each

sample adequately using a vortex mixer before pipetting. Followed the procedure from a

to d as given below.

a) Internal standard spiking solution (25µl) was transferred in micro tube except in blank

Collision energy : 12 V (For GBP), 15 V (For LEV)

Collision gas pressure : 1.5 mTorr

MS acquire time : 3.00 min.

No of segments : 1

Segment time : 3.00 min.

Chrom filter : 10 sec

Peak width : 0.01 for GBP & LEV.

No of scan events : 2

Scan event – 1 (For GBP)

Polarity : Positive

Data type : Centroid

Scan type : SRM (Selected Reaction monitoring)

Parent Product Width Time CE Q1PW Q3PW

172.15 154.15 0.010 0.200 12 0.70 0.70

Scan event - 2 (For LEV)

Polarity : Positive

Data type : Centroid

Scan type : SRM (Selected Reaction monitoring)

Parent Product Width Time CE Q1PW Q3PW

171.13 126.06 0.010 0.200 15 0.70 0.70

Where,

Parent : Parent ion

Product : Daughter ion being monitored

Width : Scan width

Time : Scan time for event

CE : Collision energy

Q1PW : Resolution for first quadrupole

Q3PW : Resolution for third quadrupole

Divert Valve Settings : Not in use.

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Samsung RT34M Uživatelský manuál Strana 52