Samsung RT34M Uživatelský manuál stáhnout pdf (Strana 25)

Dushyant Patel Review of Literature

S.K.P.C.P.E.R., Kherva 13 M .Pharm. Thesis

21. Spectrofluorimetric determination

of vigabatrin and gabapentin in

dosage forms and spiked plasma

samples through derivatization with

4-chloro-7-nitrobenzo-2-oxa-1,3-

diazole

22

.

Spectrofluor

imetry

Derivatization reagent:

4-chloro-7-nitrobenzo-2-oxa-

1,3-diazole

Detection method:

Fluorimetry

(EX: 465 nm, EM: 532 nm)

22. Colorimetric determination of

gabapentin in pharmaceutical

formulation

23

.

Colorimetry Based on following reaction

1) GBP with vanillin

(Duquenois reagent) in the

presence of McIlvain buffer

pH 7.5 and the color

developed was measured at

376 nm.

2) The primary amino group of

GBP with ninhydrin reagent

in N, N’-dimethylformamide

(DMF) medium producing

colored product which

absorbs maximally at 569

nm.

3) The reaction of gabapentin

with p-benzoquinone (PBQ)

to form a colored product

with λmax at 369 nm.

23. Determination of the antiepileptics

vigabatrin and gabapentin in dosage

forms and biological fluids using

Hantzsch reaction

24

.

Colorimetry Reaction: condensation of the

drugs through their amino

groups with acetylacetone and

formaldehyde according to

Hantzsch reaction yielding the

highly fluorescent dihydro-

pyridine derivatives.

Color developed: yellowish

orange measured at 410 nm &

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