Samsung RT34M Uživatelský manuál stáhnout pdf (Strana 51)

Dushyant Patel LC-MS/MS Method

S.K.P.C.P.E.R., Kherva 39 M .Pharm. Thesis

4.2.11 DETECTOR PARAMETERS:

: Length x i.d. (mm) : 150 x 4.6

: Particle size (µ) : 5.0

Mobile phase : Methanol: 5 mM Ammonium formate (80:20, % v/v)

Column Temp. : 45°C

Flow rate : 1.0 ml/min with splitter. ( 30% to source & 70% to waste)

Back pressure : 140 bar (approximately)

Retention times (min) : *

GBP : 1.66 min (± 0.5 min)

LEV : 1.66 min (± 0.5 min)

Run time : 3.00 min

• Retention time of analyte and internal standard may vary between run/batch.

Shimadzu LC method parameters

Pump : Shimadzu LC-10ADvp

Pressure range : 0- 410 Bars

System controller

Model : SIL-HTc

Rinse volume : 1000 µl

Needle stroke : 52 mm

Rinse speed : 35 µL/sec

Purge time : 1.0 minutes

Rinse dip time : 0.0 sec

Rinse mode : Before and After aspiration.

Cooler enabled : Yes

Cooler temperature : 5°C

Ion source parameters :

Ion source : ESI mode

Spray voltage : 4000 v

Sheath gas : 55 (arb)

Auxiliary gas : 30 (arb)

Capillary temperature : 390 °C

Tube lens offset* : Tuned value 78.59 V (For GBP)

: Tuned value 66.83 V (For LEV)

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Samsung RT34M Uživatelský manuál Strana 51