Samsung RT34M Uživatelský manuál stáhnout pdf (Strana 49)

Dushyant Patel LC-MS/MS Method

S.K.P.C.P.E.R., Kherva 37 M .Pharm. Thesis

4.2.8 METHOD DEVELOPMENT TRIALS:

TABLE NO: 4.9 METHOD DEVELOPMENT TRIALS

SR

NO

TRIAL COLUMN MOBILE

PHASE

SAMPLE OBSERVATION

1. TRIAL-1 Betabasic C8,

50 × 4.6 mm,

5 µ

ACN: 10 mM

Amm. formate

( 80:20)

Aqueous

MQC

peak shape was not

good

2. TRIAL-2 Betabasic C8,

50 × 4.6 mm,

5 µ

ACN: 5 mM

Amm. formate

( 80:20)

Aqueous

MQC

IS peak shape was

not good

3. TRIAL-3 Betabasic C8,

50 × 4.6 mm,

5 µ

ACN: 1 mM

Amm. formate

( 80:20)

Aqueous

MQC

peak shape was

good , but higher

tailing observed

4. TRIAL-4 Betabasic C8,

50 × 4.6 mm,

5 µ

ACN: Methanol:

5 mM Amm.

formate

( 60:20:20)

Aqueous

MQC

peak shape was

good , but higher

tailing observed

5. TRIAL-5 Betabasic C8,

50 × 4.6 mm,

5 µ

ACN: Methanol:

1 mM Amm.

formate

( 60:20:20)

Aqueous

MQC

peak shape was

good, but higher

tailing was

observed

6. TRIAL-6 Betabasic C8,

50 × 4.6 mm,

5 µ

ACN: Methanol:

1 mM Amm.

formate

( 40:40:20)

Aqueous

MQC

peak shape was

good, slight higher

tailing was

observed

7. TRIAL-7 Symmetry C8,

150 × 4.6 mm,

5 µ

ACN: Methanol:

1 mM Amm.

formate

( 40:40:20)

Aqueous

MQC

peak shape was

good, slight less

tailing was

observed

8. TRIAL-8 Symmetry C8,

150 × 4.6 mm,

5 µ

Methanol :

1 mM Amm.

formate

( 80:20)

Aqueous

MQC

peak shape was

good, tailing was

not observed

9. TRIAL-9 Symmetry C8,

150 × 4.6 mm,

5 µ

Methanol :

1 mM Amm.

formate

(80:20)

Aqueous

MQC &

External

spiked

MQC

peak shape was

good, ion

suppression was

observed

1 2 ... 44 45 46 47 48 49 50 51 52 53 54 ... 92 93

Komentáře k této Příručce

Žádné komentáře