Samsung RT34M Uživatelský manuál stáhnout pdf (Strana 46)

Dushyant Patel LC-MS/MS Method

S.K.P.C.P.E.R., Kherva 34 M .Pharm. Thesis

4.2.5 PREPARATION OF CALIBRATION STANDARD AND QUALITY CONTROL

SPIKING SOLUTIONS:

TABLE NO: 4.6

Volume of GBP

intermediate stock

solution (0.50 mg/ml)

used (ml)

Diluent

up to (ml)

Spiking stock solution

GBP Conc. (µg/ml) Sample Name

0.02 10 1.00 CSS-01

0.04 10 2.00 CSS-02

0.12 10 6.00 CSS-03

0.24 10 12.00 CSS-04

0.48 10 24.00 CSS-05

0.96 10 48.00 CSS-06

1.44 10 72.00 CSS-07

1.92 10 96.00 CSS-08

2.40 10 120.00 CSS-09

0.06 10 3.00 LQCS

0.80 10 40.00 MQCS

1.80 10 90.00 HQCS

2.40 5 240.00 2ULOQCS

4.2.6 PREPARATION OF BIOLOGICAL MATRIX CALIBRATION AND QUALITY

CONTROL SAMPLES IN MATRIX:

¾ The blank biological matrix (plasma) taken out from –20°C/-70°C deep freezer and kept

at room temperature for thawing and vortexed adequately before pipetting.

a) Preparation of blank plasma:

¾ To analyte free plasma (95 µl) in micro tube diluent (5 µl) was added and vortexed to

mix. Diluent (25 µl) was added and followed the sample treatment procedure as per

section 4.2.12.

b) Preparation of zero standard:

¾ To analyte free plasma (95 µl) in micro tube diluent (5 µl) was added and vortexed to

mix. Internal standard spiking stock solution (25 µl) was added and followed the sample

treatment procedure as per section 4.2.12.

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Samsung RT34M Uživatelský manuál Strana 46